Separation of high-value extracts from Silybum marianum seeds: Influence of extraction technique and storage on composition and bioactivity

Extracts from Silybum marianum seeds have high industrial potential due to their application as phytopharmaceuticals and food. In order optimize its production, conventional extraction (in Soxhlet apparatus using solvents ethanol n-hexane) supercritical fluid (using CO2 at the pressure of 30 MPa temperature 40 °C with without co-solvent ethanol, oil-rich defatted seeds) were compared. addition, effect plant cultivation (years 2011 2019) extract storage on yield quality extracts assessed. It was shown that unsaturated fatty acids (linoleic oleic being most dominant) constituted 64–87% obtained extracts. Content ?-tocopherol varied 0.01 13 g/100 g, while total phenolic content in range 13.2 104.2 gGAE/100 gextract. vitro cytotoxic activity analysis confirmed showed against cancer cells. This study pointed out important role year, selection technique solvent, well yield, chemical profile,